Immunofluorescence Double Staining Protocol Parallel Approach

1. Preparation of Slides    A. Cell Lines Grow cultured cells on sterile glass cover slips or slides overnight at 37 º C  Wash briefly with PBS Fix as desired. Possible procedures…

Protocol For Cryosectioning

While the timing of the various steps in this protocol are probably not critical, I tend to prefer to process the tissue all at once to ensure that RNA and/or proteins do not get degraded.   Solutions 20% Paraformaldehyde/4%…

Purification Of Mitochondrial Dna

The basic procedure for breaking cells with glass beads as described in the reference Lang et al. (1977) is as follows.   MtDNA from Protists A minimum of 2-3 g wet weight of cells are necessary for one DNA extraction…

Screening And Profiling Protein Expression In Human Cancer Serum Using Antibody Array Technologies

Antibody arrays have been a promising and inexpensive tool for bulk analysis of protein level changes in human plasma and serum. These analyses have lead to proteomic profiling of a number of disease states, as well as biomarker discovery.…

Expression Levels Of The Predicted Proteins By Western Blotting

To evaluate the prediction accuracy of DAMAPEP, expression levels of the 10 proteins predicted with the 2.0 cutoff value were examined by Western blotting. Among the seven proteins predicted with increased expression in cancer cells, five proteins,…

Identifying The Differentially Expressed Proteins Between Normal And Cancer Cell Lines By Damapep

The expression profiles of 312 proteins obtained by the DAMA staining for 10 breast cell lines were repeated at least twice and were analyzed by the programs ScanAlyze and DAMAPEP. When using a minimum cutoff value of 2.0 for the absolute value…