Endnote Cheet Sheet

EndNote Cheet Sheet [fav]

Arabidopsis Seed Collection

[fav] Arabidopsis Seed Collection Luca Comai has devised a simple seed collector suitable for high density use (many plants in a small space). It is effective, very cheap, and easy to construct. The instructions for constructing it are given…

Dna Extraction Procedure From Human Blood

[fav] 1. Draw 5 ml of blood using lavender-top Vacutainer (Beckton-Dickinson; EDTA anticoagulant) 2. Keep cool until prep is performed (e.g. in an ice chest), but DO NOT FREEZE. Highest yield will be achieved by extracting within 24 hours. 3.…

Inmproving Infection Efficiency Via Virus Centrifugation

1. Ultracentrifuge the virus at 50,000 x g for 90 min at 4°C. Remove the supernatant. Drain carefully and well (preferably with a pipet) since the viral pellet is glassy and will be a filmy smear on the side of the tube.   2.…

Immunofluorescence Double Staining Protocol Parallel Approach

1. Preparation of Slides    A. Cell Lines Grow cultured cells on sterile glass cover slips or slides overnight at 37 º C  Wash briefly with PBS Fix as desired. Possible procedures…

Protocol For Cryosectioning

While the timing of the various steps in this protocol are probably not critical, I tend to prefer to process the tissue all at once to ensure that RNA and/or proteins do not get degraded.   Solutions 20% Paraformaldehyde/4%…