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Protocolpedia - The Encyclopedia Of Lab Protocols

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Farnham lab,UC Davis    Roe's lab,Univ of Oklahoma     Dr. Roth, UC Davis     Dr. Hennighausen,NIH/NIDDK     Dr. Shivdasani,Harvard Medical school     Dr. Mirmira University of Virginia     Dr. Herman, Kansas State Univ     Dr. Shiraishi,Kyoto University     Dr Pikaard Indiana Univ                            

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Bleeding Time Test
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OUTLINE

Although in the Review Article : "Idiopathic Thrombocytopenic Purpura: A practice Guideline Developed by Explicit Methods for the American Society of Hematology" bleeding time is considered as unnecessary/inapropriate to establish the diagnosis of ITP it's still used in experimental models of ITP study as an additional test.

PROTOCOL
  1. Anesthesize a mouse
  2. Make a standard incision on the dorsal tail vein using Simplate blades
  3. Immerse the tail in NaCl 0.9% at 37.5ѓC
  4. Record the time from the moment blood is observed to emmerge from the wound until cessation of blood flow
ADDITIONAL INFO
Alternatively you can perform a standard incision with an automatic device (Surgicutt; ITC, Edison, NJ) and every 15 secs apply a filter paper (Whatman 3MM) for 5 sec until the blood no longer stained it.

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