I have tried to express my protein - sucrose synthase from rice, in [/I]P.pastoris. Although I tried both electroporation and " Easy Comp" from Invitrogen, I could not get over expression of my protein.
Here are my inducing conditions:
medium BMGY FOR 16~18hr
change to BMMY OD600=1
used 0.5% methanol for induction
I am curious that should I try higher methanol for induction or try to incubate in different temperature?
Or how can I get an over expression of my protein?