The why, how and what to do of generating your own antibody. Basics and practical aspects.

One of the key factors determining good western blot is in protein sample preparation. Where proteins can be obtained from nature, lab production, or extracted cells or tissues, proper preparation is compulsory. Extraction process starts from lysing cells or tissues using lysis buffer.

Amplifying GC rich regions can be troublesome and its useful to understand why its so and what can help.